CYCLODEXTRIN GLYCOSYLTRANSFERASE - Avhandlingar.se

ALKYL GLYCOSIDE - Dissertations.se

GO - Molecular function i. alpha-amylase activity Source: InterPro 2021-02-20 2002-09-12 Cyclodextrin glucanotransferases (CGTase; E.C 2.4.1.19) belonging to the glycoside hydrolase family 13 (GH 13) are widely used as catalysts in starch conversion processes (Han et al. 2014). CGTases mainly perform three different reactions in addition to hydrolysis, namely … The product specificity and pH optimum of the thermostable cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacterium thermosulfurigenes EM1 was engineered using a combination of x-ray crystallography and site-directed mutagenesis. 2014-12-15 γ-CGTase activity increased, in both control cultures (from 71.7 to 97.0%) and cultures supplemented with β-cyclodextrin (from 56.3 to 91.8%). More importantly, the effect of β-cyclodextrin on total soluble γ-CGTase activity, as a multiple of the control value, increased 2008-08-26 The purified CGTase has a pI value of 4.2. The optimum pH of 6.0 and 60 °C temperature were found to be the best for CGTase activity.

Cgtase

First starch is liquified either by heat treatment or using α-amylase, then CGTase is added for the enzymatic conversion. Immobilisation of CGTase for continuous production of long-carbohydrate-chain alkyl glycosides Control of product distribution by flow rate adjustment. / Svensson, David; Adlercreutz, Patrick. In: Journal of Molecular Catalysis B: Enzymatic, Vol. 69, No. 3-4, 2011, p. 147-153. Research output: Contribution to journal › Article 2018-08-31 · CGTase is an industrially important enzyme for α-, β- or γ-cyclodextrins (CDs) production, which are extensively used in agriculture, chemicals, cosmetics, foods and pharmaceuticals .

Screening och val av medelkomponenter för cyklodextrin

In this work, the CGTase produced by Bacillus clausii strain E16 was used to produce CD from maltodextrin and different starches (commercial soluble starch, corn, cassava, sweet potato, and waxy corn starches) as substrates. The E-domain of cyclodextrin glycosyltransferase (CGTase) (EC 2.4.1.19) from Bacillus circulans strain 251 is a putative raw starch binding domain. Analysis of the maltose-dependent CGTase crystal structure revealed that each enzyme molecule contained three maltose molecules, situated at contact poi ….

Cloning and expression of a putative cyclodextrine

(Castillo et al., 2018). 1996-01-01 · This chapter discusses the purification and action of cyclodextrin-producing enzyme (CGTase). Purification of macerans CGTase can be accomplished by following steps: Starch adsorption and desorption, column chromatography on diethylaminoethyl-cellulose, and crystallization. By use of the crystal CGTase preparation, the action of the enzyme is studied. Purpose γ-Cyclodextrin glycosyltransferase (γ-CGTase) catalyzes the biotransformation of low-cost starch into valuable γ-cyclodextrin (γ-CD), which is widely applied in biotechnology, food, and pharmaceutical industries. However, the low specificity and activity of soluble γ-CGTase increase the production cost of γ-CD, thereby limiting its applications. Therefore, the present study aimed 2009-07-08 · Activity characteristics and kinetic aspects of a cyclodextrin glycosyltransferase (CGTase) from Bacillus circulans DF 9R were studied.

Oct 25, 2018 Abstract Cyclodextrin glucanotransferases (CGTases) synthesize cyclic oligosaccharides (cyclodextrins, CD) from starch. A CGTase from The results suggest that the overexpression level of recombinant CGTase excreted into the culture medium using the recombinant Escherichia coli could be In enzymology, a cyclomaltodextrin glucanotransferase (also cyclodextrin glycosyl transferase or CGTase for short) (EC 2.4.1.19) is an enzyme that catalyzes the 17 matches Synonym: FTase Inhibitor I - Calbiochem · Empirical Formula (Hill Notation): C22 H38N4O3S Molecular Weight: 470.69. Nov 12, 2019 ABSTRACT.
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By use of the crystal CGTase preparation, the action of the enzyme is studied. Purpose γ-Cyclodextrin glycosyltransferase (γ-CGTase) catalyzes the biotransformation of low-cost starch into valuable γ-cyclodextrin (γ-CD), which is widely applied in biotechnology, food, and pharmaceutical industries.

p.232-235 Fig. 1 Effect of chemical chaperone candidates on γ-CGTase expression in E. coli. a Comparison of soluble γ-CGTase activity and cell growth in shake-flask cultures supplemented with different chemical chaperone candidates. Bars represent extracellular γ-CGTase activity (black), soluble periplasmic γ-CGTase activity (light gray), and DCW (gray).
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Kina Alpha Cyclodextrin CAS-nr 10016-20-3

Alexander von Humboldt-Stiftung Humboldt-Stipendiaten aus

17·1 was cloned in Escherichia coli Jan 22, 2016 A CGTase catalyzing disproportionation of the linear maltooligosaccharide chain formed in the initial coupling reaction might utilize a greater Buy CGTase recombinant protein, Cyclomaltodextrin glucanotransferase, partial Recombinant Protein-P30920.1 (MBS1124191) product datasheet at Cyclodextrin glucanotransferases (CGTases) convert starch to cyclodextrins (CD) of various sizes. To engineer a CGTase for the synthesis of large-ring CD TY - JOUR.

CGTase enzyme production is depicted in Figs 2 and 3 for Bacillus sp.