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KEYWORDS: Micro-PIV, Ultrasound Resonances, Acoustophoresis, Cell Handling INTRODUCTION Microchannel acoustophoresis is a rapidly expanding research ﬁeld allowing gentle and efﬁcient manipulation of cells and other biological particles [1, 2]. The larger cancer cells are acoustophoretically driven into the center stream by the radiation force, whereas white and red blood cells do not migrate as far in the acoustic field and thus exit through the side outlets. Similar rates of cancer-cell recovery, cancer-cell purity, and fold-enrichment were seen with both prostate cancer (DU145, PC3) and breast cancer (MCF7) cell line cells. We identified eosinophil granulocytes as the predominant white blood cell (WBC) contaminant (85%) in the enriched cancer-cell fraction.
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However, more recent developments have This ultrasonic standing wave-based technology, iso-acoustophoresis, offers This enables access to viable and unaltered circulating tumor cells, important for The Josefsson prostate cancer group is a multi-disciplinary bench-to-bedside research team in the Wallenberg Centre for Molecular Medicine. We aim to NK cells (orange) attacking an ultrasound-fabricated solid tumor (green) of particles and biological cells (acoustophoresis, acoustic trapping) in Cytometry and Biomedical Applications: Multidimensional Acoustophoresis Ultrasound-assisted Interactions of Natural Killer Cells with Cancer Cells and Swedish University dissertations (essays) about ACOUSTOPHORESIS. cell transplantation has developed as a standard treatment for many cancers besides development of several novel therapies for castrate-resistant prostate cancer, ultrasonic wave based microfluidic techniques (acoustophoresis) to enable. Umeå universitet, får 820 000 kronor.
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Acoustophoresis. Our approach to separate CTCs from white blood cells is using a label-free ultrasound standing wave-based microfluidics platform called Acoustophoresis (Magnusson et al 2017, Antfolk et al 2015, Augustsson et al 2012). This cell separation is based on intrinsic cellular properties such as size, density and compressibility.
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In Proceedings of the 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 127-129) Projekt: Apheresis-acoustophoresis to improve cancer diagnosis in prostate cancer (APACA-PC). Anna Lantz, lnst för Medicinsk Epidemiologi och Biostatistik, Karolinska Institutet, får 388 784 kronor. Projekt: Evaluation and optimization of active surveillance for men living with clinically insignificant prostate cancer. Researchers from MIT, Pennsylvania State University, and Carnegie Mellon University have devised a new way to separate cells by exposing them to sound waves They have presented an acoustophoresis device with trifurcation inlet and outlet to separate three different prostate cancer cell lines (DU145, PC3, and LNCaP) from no-cancer control subjects’ blood sample spiked with the mentioned cancer cells . Second part of the thesis focuses on acoustophoresis. In (paper 5), a multifunctional acoustic microdevice was developed for isolation of cancer cells from red blood cells with a separation efficiency of 92.4% and trapping efficiency of 93%.
these challenges, a new technology that sorts blood cells using acoustic waves, referred to as acoustophoresis, is being developed. Benefits
”Miniaturised and Quantitative Techniques Targeting Prostate Cancer” “On microchannel acoustophoresis – Experimental considerations and life science
tillämpningar inom cancer upptäckt och individualiserad medicin. Huang, T. J. Surface acoustic wave (SAW) acoustophoresis: now and
Blodet och immunförsvaret Cancer Feature Blood Progenitor Cell Products Using a Novel Micro-Chip Based Acoustophoretic Platform. Post-transcriptional control of stem and cancer cell fate.
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This cell separation is based on intrinsic cellular properties such as size, density and compressibility. The aim of this thesis was to compare different ways of dissociating tissue - specifically breast cancer tissue - and sorting out tumour cells and immune cells from the dissociated tissue using acoustophoresis in a microfluidic chip. Microfluidic, Label-Free Enrichment of Prostate Cancer Cells in Blood Based on Acoustophoresis Forskningsoutput : Tidskriftsbidrag › Artikel i vetenskaplig tidskrift Översikt Using this microchannel configuration, several publications have shown cancer cell lines (breast and prostate) being efficiently separated from WBCs from RBC-lysed blood using acoustophoresis with BASWs [3, 20].This label-free separation occurs because larger-sized cancer cells focus faster to the center pressure node than WBCs in the separation channel; thus, if flow rates are optimized the ACOUSTOPHORESIS -A SOUND APPROACH TO CHIP BASED CELL HANDLING – T. Laurell1,2 1 Dept. Measurement Science and Industrial Electrical Engineering Div. Nanobiotechnology, Lund University, Lund, SWEDEN 2 Dept.
The single inlet approach makes this instrument insensitive to acoustic impedance mismatch; a phenomenon reported to importantly affect accuracy in multi-laminar flow stream acoustophoresis. Request PDF | Acoustophoresis in Tumor Cell Enrichment | This chapter outlines the opportunities and current developments of label-free separation of circulating tumor cell (CTC) from blood cells
Label-free concentration of viable neurons, hESCs and cancer cells by means of acoustophoresis† Marina C. Zalis , a Juan F. Reyes , b Per Augustsson , c Staffan Holmqvist , d Laurent Roybon , d Thomas Laurell c and Tomas Deierborg * a
Cancer cells display acoustic properties enabling acoustophoretic separation from white blood cells (WBCs) with 2–3 log suppression of the WBC background. However, a subset of WBCs has overlapping acoustic properties with cancer cells, which is why label-free acoustophoretic cancer cell isolation needs additional purification prior to analysis.
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Acoustic Forces in Cytometry and Biomedical Applications
Our results show that the three-step acoustophoresis method can be used for on-chip sample prep-aration in applications using uorescence-based cellular analysis. Materials and methods Microbead suspensions For nonfixed, viable cells, tumor cell recovery ranged from 72.5% to 93.9% with purity ranging from 79.6% to 99.7%. These data contribute proof-in-principle that label-free microfluidic acoustophoresis can be used to enrich both viable and fixed cancer cells from WBCs with very high recovery and purity. design and use of acoustophoresis micro-devices for long-term cell handling, and confirm that standing-wave ultrasound is a suitable cell manipulation technology for clinical applications even at high pressure amplitudes.
Benefits ”Miniaturised and Quantitative Techniques Targeting Prostate Cancer” “On microchannel acoustophoresis – Experimental considerations and life science tillämpningar inom cancer upptäckt och individualiserad medicin. Huang, T. J. Surface acoustic wave (SAW) acoustophoresis: now and Blodet och immunförsvaret Cancer Feature Blood Progenitor Cell Products Using a Novel Micro-Chip Based Acoustophoretic Platform. Post-transcriptional control of stem and cancer cell fate.
cancer cells and A498 renal carcinoma cells.